Promoters that were differentially methylated in all LS tumor groups but not in FAP adenoma vs. FAP normal or LS normal vs. FAP normal comparisons were ADHFE1, EYA4, FLI1, GRIA4, KLF17, LBX2, PREX2, RIMS1, and ZNF528. This evidence concerns the gene EYA4 and Leigh syndrome.