Promoters that were differentially methylated in all LS tumor groups but not in FAP adenoma vs. FAP normal or LS normal vs. FAP normal comparisons were ADHFE1, EYA4, FLI1, GRIA4, KLF17, LBX2, PREX2, RIMS1, and ZNF528. The gene discussed is RIMS1; the disease is Leigh syndrome.