The regions with more than 10 CpGs were overlapping with PITX1, VELF2, FBLIM1, OR2I1P, OPLAH, and TXNRD1, EID3. Notably, of these largest DMRs, FBLIM1, OR2I1P, and OPLAH were identical or represented the same but somewhat smaller region than observed in all LS tumor groups (Additional Table 6), providing further support for a general role in CRC tumorigenesis. Here, PITX1 is linked to Leigh syndrome.