CAMK2D and cardiac hypertrophy: As examples of differentially phosphorylated proteins seven-days post-TAC, we visualised the differential phosphorylation sites within MYL2 (Myosin Light Chain 2), a sarcomere protein with important roles in cardiac contraction and cardiomyopathies, and CAMK2D (calcium/calmodulin-dependent protein kinase type 2 d), the major CAMK2 isoform expressed in the heart playing key roles in calcium homeostasis in cardiomyocytes and linked to the decompensation from cardiac hypertrophy to heart failure (Fig. 3d)40–42.