We collected PBMCs from 12 metastatic melanoma patients, stimulated them in vitro with the selected HLA-A *0201 epitopes and measured specific CD8 T-cell responses following restimulation either by IFNγ production (SF15-dec1 and GV17p) or tetramer staining (VS17p). The gene discussed is HLA-A; the disease is metastatic melanoma.