To investigate the potential roles of human gut microbiota in the association of TRPM7 genotype, high Ca:Mg intake ratios, and colorectal adenomas and serrated polyps, we performed whole genome sequencing on stool, rectal swab, and mucosal tissue samples of participants in the Personalized Prevention of Colorectal Cancer Trial (PPCCT) designed to examine the interaction between Ca:Mg intake ratios and the TRPM7 genotype in affecting biomarkers for colorectal carcinogenesis. This evidence concerns the gene TRPM7 and colorectal cancer.