As further confirmation, infection of NLRP3-deleted THP-1 cells (Fig. 2d) and BMDMs (Fig. 2e) completely prevented IL-1β secretion, while preserving secretion of the inflammasome-independent cytokine TNFα (Fig. 2f), highlighting the specificity of the effect for inflammasome-dependent responses. The gene discussed is IL1B; the disease is infection.