To further quantification of CD80 expression, we conducted the flow cytometry analyses of cell surface CD80 and the results indicated that FLT3-ITD AML cells (MV4-11) displayed remarkable CD80 cell surface expression, but not the FLT3-WT leukemic cells (RS4;11) (Figure 1B). The gene discussed is FLT3; the disease is acute myeloid leukemia.