To confirm platelet-specific gene deletion, we used flow cytometry analysis and found successful deletion of integrin-β3 on CD41+ platelets in both blood and intestinal cells of TNBS-induced NEC-like injury in integrin β3fl/fl PF4-Cre + (integrin-β3−/−) murine pups than integrin β3fl/wtPF4-Cre + (integrin β3+/−) (Figure 3B). The gene discussed is PF4; the disease is necrotizing enterocolitis.