ITGA2B and necrotizing enterocolitis: Regarding the time kinetics of platelet activation during NEC-like injury, we investigated blood samples collected at various time points (0, 3, 9 and 24 h) after TNBS induction using flow cytometry analysis, which showed that activation occurs between 3 and 9 h with higher expression of integrin-β3 (identified by using clone JON-A antibody) on CD41+ platelets and steadily increased until 24 h (Figure 2D).