A greater percentage of platelet-monocyte aggregation (CD41+ Ly6C+) was observed in blood (0.62 ± 0.02% of cells in NEC-like injury controls vs. 0.11 ± 0.005% of cells in NEC-like injury induced integrin-β3−/−; P < 0.001) and intestine cells (3.00 ± 0.03% of cells in NEC-like injury controls vs. 0.20 ± 0.004% of cells in NEC-like injury induced integrin-β3−/−; P < 0.001) of control PF4-Cre+ mice after TNBS-induced NEC-like injury, whereas integrin β3−/− murine pups showed significantly reduced platelet-monocyte aggregates after TNBS-induction (Figure 3C). This evidence concerns the gene PF4 and necrotizing enterocolitis.