In the CL model by L. braziliensis, we observed an anti‐inflammatory effect, potentially caused by the significant increase in CD4+LAP+ and CD4+CD25+Foxp3+ T cell frequencies in draining lymph nodes from 6 to 8 weeks post‐infection in mice from Lb/HSP65 compared to Lb and Lb/Ø groups. This evidence concerns the gene HSPD1 and infection.