Prime editors have consistently demonstrated genomic correction rates below 10%, with specific examples including 6.4% correction in RPE tissue derived from a model of Leber congenital amaurosis and 1.71% correction in the Dnmt1 gene90 In terms of specificity, key future challenges for prime editing will be off-target effects, which are unintended modifications at genomic sites other than the intended target. This evidence concerns the gene DNMT1 and Leber congenital amaurosis.