To explore the molecular mechanisms by which hepatocyte-derived SERPINA3 or Serpina3k mitigates liver fibrosis, we produced CM from AML12 cells transfected with control, mSerpina3k-GFP or hSERPINA3-GFP, which were then applied to HSC-T6 rat HSCs (Fig. 6a, b). Here, SERPINA3 is linked to Hepatic fibrosis.