Immunostaining further confirmed infection of pyramidal neurons in the hippocampus (Figure 4(E), Supplementary Fig. 4, r = 0.603, M1 = 0.796, M2 = 0.648) and cerebral cortex (r = 0.527, M1 = 0.851, M2 = 0.489), whereas Purkinje cells in the cerebellum were confirmed to be infected through visual overlap of tGFP signals with calbindin-positive staining (Figure 5). This evidence concerns the gene CALB1 and infection.