In order to determine how sucralose affects the tumor microenvironment, we performed single-cell RNA sequencing on 76,804 cells isolated from the tumors (41,850 cells) and tumor-draining lymph nodes (tdLN; 34,954 cells) of sucralose-consuming, anti–PD-1–treated Taconic mice. The gene discussed is PDCD1; the disease is neoplasm.