Further analysis of CD8+ T cells from the tumor and tdLN of sucralose + anti–PD-1 mice showed an increase in T-cell exhaustion signatures, including increases in Pdcd1, Tox, Lag3, Tigit, Icos, Ctla4, and Prf1, as well as downregulation of some solute carrier (SLC) family members, including those that transport glucose (Slc2a3 and Slc2a1) and those required for mitochondrial respiration (Slc25a36 and Slc25a19; Fig. 3C and D; Supplementary Fig. S6A–S6E). This evidence concerns the gene ICOS and neoplasm.