Here, we demonstrate anti-CD16-EGFR BICA produces an NK-mediated cytotoxic effect when expressed in various cancer cell lines in vitro, although the resulting NK-mediated tumor cell killing is not as potent as seen with the CD3-BICA in replication-deficient transduced cells; we see complete killing with the addition of the oncolytic (Figure 4). Here, EGFR is linked to cancer.