No significant associations were observed between the post-to-pre-treatment CD8+ count ratio and variables such as sex, radiotherapy approach (SCRT vs. LCRT), LVI status, PNI status, mucinous versus non-mucinous tumor histology, clinical node status, clinical T and N stages, ypT stage, ypN stage, TRG, or surgical grade. This evidence concerns the gene CD8A and mucinous neoplasm.