⁃ HIIT and MICT both significantly reduced serum myonectin.⁃ Myonectin levels in muscle tissue decreased only with HIIT.⁃ Liver tissue myonectin increased with NAFLD induction but did not significantly change with exercise.⁃ No significant difference between HIIT and MICT in serum or tissue myonectin levels. This evidence concerns the gene C1QTNF5 and metabolic dysfunction-associated steatotic liver disease.