This upregulation was further confirmed by qRT-PCR analysis, which demonstrated significantly higher PLA1A expression in CRC cell lines (HT-29, SW480, LOVO, and SW620) compared to normal human colon mucosal epithelial cell line (NCM460), with the highest expression observed in the SW620 cell line (P < 0.05, Figure 10D). Here, PLA1A is linked to colorectal carcinoma.