To verify the existence of a stromal-localized immunosuppressive network coordinated by Macro_SPP1/C1QC macrophages and CD8_Tex_C1 exhausted T cells in gastric cancer tissues, and to investigate the correlation between MIF expression and immunotherapy efficacy, we performed tissue immunofluorescence staining on gastric cancer biopsy specimens. The gene discussed is SPP1; the disease is gastric cancer.