These two subpopulations were mediated by distinct transcriptional regulatory networks (Tex_C1: TEAD1/KLF13; Tex_C2: E2F family), and their spatial distribution showed an evolutionary trend from the tumor periphery stroma (Tex_C1) to the tumor core (Tex_C2), revealing the coupling between exhaustion and T cell migration (49). The gene discussed is TEX46; the disease is neoplasm.