The pathways enriched included systemic lupus erythematosus, vibrio cholerae infection, biosynthesis of various other secondary metabolites, isoflavonoid biosynthesis, prolactin signaling pathways, and nonribosomal peptide structures in healthy tomatoes treated with Cu-Ag nanoparticles and thiodiazole-copper. Here, PRL is linked to systemic lupus erythematosus.