Collectively, this study elucidates for the first time that acetate, as a key gut microbiota metabolite, exerts anti‐EMs effects by activating the JAK1/STAT3 signalling pathway to drive macrophage reprogramming toward the M1 phenotype, thereby positioning gut microbiota reconstruction as a novel therapeutic strategy for endometriosis. This evidence concerns the gene STAT3 and endometriosis.