Our pan-cancer analysis indicated a statistically significant expression of the PFKFB4 gene in various tumor types, including adrenal, acute myeloid leukemia (AML), bladder, breast, colon, esophageal, liver, lung squamous cell carcinoma, ovarian, pancreatic, rectal, renal clear cell carcinoma, renal chromophobe, renal papillary, skin, stomach, testicular, as well as in both endometrial and cervical uterine cancers. Here, PFKFB4 is linked to squamous cell lung carcinoma.