TTR and neoplasm: To control for baseline tumour burden, mice were stratified by plasma AFP levels at 28 weeks, and two groups with equivalent AFP levels were injected with hepatocyte-targeted adeno-associated virus 8 (AAV8)-TTR vectors expressing either YFP (wild type (WT)) or Cre recombinase (Acly knockout (KO)) to induce hepatocyte-specific Acly deletion (Fig. 1a and Extended Data Fig. 2a,b).