Last, we found that deletion of p16-positive cells decreased the levels of SASPs, including IL-1β、IL-6、MCP-1, and TNF-α, in AP20187-treated DM INK-ATTAC kidneys as analyzed with qRT-PCR analysis (Fig. 4C), which might contribute to the downregulation of KIM-1 and other factors to decrease renal injury in those kidneys. The gene discussed is TNF; the disease is diabetes mellitus.