We further found that the expression of the key glycolytic enzymes, Hk1, Ldha, Pkm2, and Ldhb, was increased in DM mouse kidneys compared to that in age-matched wild-type mouse kidneys, whereas deletion of p16-positive senescent cells decreased the expression of these key glycolytic genes in AP20187-treated DM INK-ATTAC kidneys (Fig. 5B). This evidence concerns the gene HK1 and diabetes mellitus.