To explore potential overlapping regulation of genes and oncogenic signaling pathways through ERK and TBK1–IKKε in pancreatic cancer, we treated MIA PaCa-2 cells for 24-h with the ERK inhibitor SCH772984 or the TBK1–IKKε inhibitor compound 1 (27) before RNA purification and RNA-Seq analysis. Here, TBK1 is linked to familial pancreatic carcinoma.