In adults aged 50−59 years, high Klotho levels (vs. low) were associated with a 45% reduction in tinnitus risk in the unadjusted model (OR=0.55, 95% CI: 0.31–0.97, p = 0.038), with a similar trend in the fully adjusted model (OR=0.55, 95% CI: 0.31–0.99, p = 0.046). Here, KL is linked to Tinnitus.