ELN and aortic aneurysm: Further work was undertaken to study miR-26b-5p localization and expression by in situ hybridization in another cohort of patients with aortic aneurysms, focusing on low (von Kossa staining 0–0.99%) and medium levels (von Kossa staining < 10%) of calcification with intact elastin.9 Analysis of the positive miR-26b-5p probe signal showed that miR-26b-5p localization significantly decreased with increasing levels of mineralization (von Kossa staining) in the aorta, importantly paralleled by a higher expression of p-SMAD1 (Figure 7C and Supplementary material online, Figure S7C and D).