The t‐SNE plots of representative marker gene expression (Figure 1B), including CD79A (B cells), CD3D (T cells), LYZ (myeloid cells), EPCAM (epithelial cells), CLDN5 (endothelial cells), COL1A1 (fibroblasts), MS4A2 (mast cells), NKG7 (NK cells) and MKI67 (proliferating cells), confirmed the presence and spatial distribution of these subsets within the tumour microenvironment. This evidence concerns the gene COL1A1 and neoplasm.