Cholic acid, a primary bile acid present in the liver, is metabolized in the intestine via both the classical pathway mediated by CYP7A1 and the alternative pathway involving CYP27A1; it subsequently activates metabolic receptors such as FXR and TGR5 in the intestine and liver, thereby regulating host metabolism in hyperlipidemia induced by HFD through the enterohepatic circulation (Li, Cui, et al. 2021; Wang et al. 2023). This evidence concerns the gene GPBAR1 and hyperlipidemia.