TNF and neoplasm: Antigen pulsing was performed by culturing the mDCs in the presence of cytokines rh-IL-4, rh-GMC-SF, rh-IL-6, rh-IL1b, rh-IL12, rh-IL-15, and rh-TNF-α and incubating 106 cells with 18 μg of tumor lysate or cultured cell lysate for 18 h (Figure 1C).