The role of TRAIL in anti-tumor cytotoxicity of PM21-NK cells towards A549 tumor cells was determined by treating PM21-NK cells with IFN-I overnight and then incubating treated PM21-NK cells with anti-TRAIL or isotype control antibodies for 1 h prior to co-culturing with A549-NLR tumor cells. This evidence concerns the gene TNFSF10 and neoplasm.