We tested STAT1 phosphorylation at different time points after FMDV infection, and the results showed that p-STAT1 could be detected in both PLA2G16-overexpressing cells and wild-type cells from 12 h to 24 h after infection, and the p-STAT1 level was significantly higher in PLA2G16-overexpressing cells than in wild-type cells (Figure 4a,b). This evidence concerns the gene STAT1 and infection.