In this study, AlCl-NOTA-iPD-L1 showed an IC50 of 9.27 ± 2.69 nM and a 4T1 tumor uptake (PD-L1 +++) of [18F]AlF-NOTA-iPD-L1 of 6.4% ± 0.9% ID/g, confirming its effectiveness and specificity in detecting PD-L1 protein. This evidence concerns the gene CD274 and neoplasm.