They prepared liposomes by thin film hydration followed by freeze-thawing and extrusion, composed of PC, SM, and ovine wool chol in a 55:30:10 weight ratio, adding different amounts of 1,2-dioleoyl-sn-glycero-3-{[N-(5-amino-1-carboxypentyl)-iminodiacetic acid]succinyl}(nickel salt), exploited to conjugate to the LUV surface APO2L/TRAIL-His10 (a TNF superfamily member, potentially useful in the treatment of autoimmune diseases). Here, TNFSF10 is linked to autoimmune disease.