Based on the ΔGbind calculation results and assuming a positive correlation between RBD-ACE2 affinity and virion transmissivity/infection, Ridgway et al. [38] extrapolated higher transmission rates (relative to the wild-type) for variants possessing one or more of the following substitutions: N501F, N501H, N501M, N501Y (Alpha, Omicron), E484Q, E484R (Delta, Kappa) or K417R, all of which exhibited gains in ACE2 binding affinity, i.e., negative ΔGbind values. The gene discussed is ACE2; the disease is infection.