As shown in Figure 6A–C and Figure 7E–F, infection with 103 TCID50 of PRV-GXLB-2013 resulted in significantly elevated levels of TNF-α, IL-1β, IL-6, IL-8, and MLKL in brain tissues, serum, and C8-D1A cells, compared to the control group (p < 0.01). This evidence concerns the gene MLKL and infection.