In this paper, we determined the preparation of the EVs by detecting the standard EVs protein markers CD63, CD81, and CD9 in serum EVs from TB patients, and we also determined the fluorescence expression of CD63, CD81, and CD9 in the TB patient group by analyzing the fluorescence intensity of them when CD63, CD81, and CD9 were used as the capture antibodies. The gene discussed is CD63; the disease is tuberculosis.