After incubation with Nby-Aby and DAB staining, we observed that in TMAs-1 samples all initially diagnosed as 0 and 1 +, Nby-Aby accurately identified cancer cells within the tissues, and upgrading HER2 IHC scores from 0 (Fig. 4A) or 1 + (Fig. 4B) to 2 + and 3 +. Here, ERBB2 is linked to cancer.