Given its central roles in maintaining cellular homeostasis, we hypothesized that DNA-PKcs is critically involved in orchestrating the intrinsic repair mechanisms of renal tubular epithelial cells following I/R injury.This research aims to explore how DNA-PKcs participates in the repair of renal tubular epithelial cell injury, with the hope of providing novel insights into potential strategies for enhancing the repair of AKI. The gene discussed is PRKDC; the disease is acute kidney injury.