PDK1 and osteosarcoma: High PDK1 expression also predicted poorer overall and metastasis-free survival in patients with osteosarcoma according to the microarray dataset (GSE21257) (Fig. 2D and Supplementary Fig. 2C) and was linked to poor prognosis of patients with soft tissue sarcoma (TCGA-SARC) (Supplementary Fig. 2D).