CD8A and metastatic malignant neoplasm in the lymph nodes: In both datasets, the CD8 TILs from lymph node-negative patients showed a significantly higher neoantigen specificity score compared to CD8 TILs from patients with lymph node metastasis (Dataset 1: p < 2.22 × 10−16; Dataset 2: p = 6.1 × 10−14; Figure 5B).