V‐ATPase, a multimeric complex comprising the cytoplasmic V1 domain and the V0 domain, has garnered sustained attention for its important role in regulating lysosomal function.[11, 21] Recently, several v‐ATPase subunits, such as ATP6L and ATP6V1A, have been suggested to alter the lysosomal function and participate in tumorigenesis.[22] However, as an important v‐ATPase assembly subunit, the biological function of ATP6V0E1 in tumor metastasis has not been explored. This evidence concerns the gene ATP6V0E1 and neoplasm.