Therefore, we intended to evaluate further and validate the prospective prognostic potential of two significant genes, EIF4G3 and SF3B1, that we previously identified with increased mRNA expression levels in precursor lesions through lavage sample testing these markers in a cohort study (new patients) and determine the risk of progression of low- and high-grade CIN lesions [10]. This evidence concerns the gene SF3B1 and cervical squamous intraepithelial neoplasia.