PACS1 and colorectal carcinoma: To evaluate the consistency of the Taqman qPCR results with the RNA-seqdata from a previous study [7] we applied a one-sample t-test (test value = 0) to assess the log2 fold changes in the cfRNA expression of HPGD, PACS1, and TDP2 in CRC patients (Figure 1a–c).