Molecular docking simulations showed thatchalcone 4AAF has high selectivity for 5-HT3A and 5-HT2C receptors with relevant statistical, energetic, and interactiondescriptors, and stimulates allosteric behavior toward the 5-HT2A receptor, where it provides enhanced modulatory activity.In addition, aminochalcone showed anxiolytic and hypoglycemic effects,reversing sucrose-induced anxiety and hyperglycemia in adult zebrafishand delaying the clonic stage of the epileptic crisis. This evidence concerns the gene HTR3A and Anxiety.