To determine the mechanisms by which natural active botanical metabolites ameliorate MAFLD through targeting the AMPK signaling pathway, Non-alcoholic Fatty Liver Disease, Metabolic dysfunction-associated fatty liver disease, NAFLD, MAFLD, AMP-Activated Protein Kinases, AMPK, Chinese herbs, Chinese medicine, Herbal medicine, Plant medicine, Natural medicine, Botanical drug, Phytomedicine were used as keywords or subject headings to search for relevant articles in the Web of Science and PubMed databases from 2019 to 2024. This evidence concerns the gene PRKAA1 and metabolic dysfunction-associated steatotic liver disease.