Building on the observed regulatory role of tRF-3021a in upregulating ADAM10 and sMICA expression in colorectal cancer (CRC) cells—where ADAM10-mediated cleavage of mMICA generates sMICA to promote tumor growth by evading NK cell immune surveillance—we further investigated the functional impact of tRF-3021a on CRC malignancy. The gene discussed is ADAM10; the disease is colorectal cancer.