We therefore next examined the effects of an inhibitor of diacylglycerol acyl transferase (DGAT), the rate‐limiting enzyme in TG synthesis, in cancer cells undergoing acute pHe swapping experiments (instead of cancer cells chronically adapted to pHe 6.5 as previously reported).[26] We found that the pHe 7.4 > 6.5 shift at the time of DHA and DGATi exposure induced dramatic cytotoxic effects while the acute pHe 6.5 > 7.4 shift prevented the development of cell toxicity (Figure 3A; Figure S3A, Supporting Information). Here, DGAT1 is linked to cancer.