However, following the repeated administration of GnRH agonists—first buserelin and later triptorelin—over a period of 10 days, estradiol levels rose to supraphysiologic levels, accompanied by abdominal bloating, abdominal pain, and enlarged ovaries with several ovarian cysts, which are clinical signs indicating a potential diagnosis of OHSS. This evidence concerns the gene GNRH1 and Ovarian cyst.