According to the 538 gene custom panel compiled from the International Union of Immunological Societies (IUIS) Expert Committee and PanelApp England (Primary immunodeficiency or monogenic inflammatory bowel disease (Version 4.0)), two hot VUS and one P variant were found (Table 2 and Supplementary Table S3):  TGFBR2 (p.R224H) in Pt#2; CARD11 (p.S1026C) in Pt#25; MEFV (p.M694V) in Pt#20. Here, MEFV is linked to inflammatory bowel disease.