Proteins unique to the U-EVs of the GS 8–9 group included cytoskeleton-related proteins such as actin cytoplasmic 2, F-actin-capping protein subunit beta, tubulin alpha, and AHNAK (desmoyokin), which plays roles in tumour suppression, immune regulation, and calcium homeostasis, as well as in EV release from carcinoma cells [28,29]. The gene discussed is ACTG1; the disease is carcinoma.