Treating four established NB cells lines (SMS-KCNR, SH-SY5Y, BE (2)-C, CHLA-90) and two primary NB cell lines with Tolcapone for 48 h decreased cell viability in a dose-dependent manner and induced cell death by activating caspase-3-mediated apoptosis in neuroblastoma cell lines [16]. The gene discussed is CASP3; the disease is neuroblastoma.